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cfse fluorescent dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cfse fluorescent dye
    Cfse Fluorescent Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfse+fluorescent+dye/fluorescent+dye+cfse/pmc12145548-136-1-1
    Average 90 stars, based on 1 article reviews
    cfse fluorescent dye - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Saline:

    Article Title: Upregulation and activation of p53 by erastin‑induced reactive oxygen species contribute to cytotoxic and cytostatic effects in A549 lung cancer cells.
    Article Snippet: .. A549 cells were washed in phosphate-buffered saline (PBS) twice and 100 μl of CFSE fluorescent dye (50 μmol/l; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was added, followed by incubation at 37 ̊C for 30 min. ..

    Incubation:

    Article Title: Upregulation and activation of p53 by erastin‑induced reactive oxygen species contribute to cytotoxic and cytostatic effects in A549 lung cancer cells.
    Article Snippet: .. A549 cells were washed in phosphate-buffered saline (PBS) twice and 100 μl of CFSE fluorescent dye (50 μmol/l; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was added, followed by incubation at 37 ̊C for 30 min. ..

    Article Title: In vitro study of interactions between silicon-containing nanoparticles and human peripheral blood leukocytes.
    Article Snippet: The effects of silicon dioxide-based nanoparticles on the viability and proliferative activity of human peripheral blood cultured lymphocytes were studied.. All nanoparticles in a concentration of 100 μg/ml produced a signifi cant cytotoxic effect, its intensity depending on particles’ structure: SiO2 nanoparticles were least toxic, while Ce3 +-intercaled montmorillonite nanoparticles were most toxic.. The cells died mainly by apoptosis and postapoptotic necrosis.

    Phagocytosis Assay:

    Article Title: A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx
    Article Snippet: .. For macrophage phagocytosis assay, macrophages were pre-incubated with Popu CATH (10 μM) for 2 h, S. aureus and E. coli bacteria were preloaded with 10 μM CFSE fluorescent dye (Molecular Probes, Invitrogen) in PBS for 30 min at 37°C. ..

    Bacteria:

    Article Title: A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx
    Article Snippet: .. For macrophage phagocytosis assay, macrophages were pre-incubated with Popu CATH (10 μM) for 2 h, S. aureus and E. coli bacteria were preloaded with 10 μM CFSE fluorescent dye (Molecular Probes, Invitrogen) in PBS for 30 min at 37°C. ..

    Labeling:

    Article Title: Defects in aminoacyl-tRNA synthetase cause partial B and T cell immunodeficiency
    Article Snippet: In vivo homeostatic proliferation assay Naïve CD44 lo LN T cells were harvested from congenic CD45.1 + B6 mice and CD45.2 + sti mice by cell sorting using BioMag goat anti-mouse IgG beads (Qiagen). .. CD45.1 + WT and CD45.2 + sti T cells labeled with CFSE fluorescent dye (Invitrogen) were mixed at a 1:1 ratio, and a total of 1 × 10 6 naïve T cells were adoptively transferred into lymphopenic RAG1 –/– host mice via intravenous injection. ..

    Article Title: Defects in aminoacyl-tRNA synthetase cause partial B and T cell immunodeficiency.
    Article Snippet: Aminoacyl-tRNA synthetases (ARSs) are emerging as important regulators in various immune diseases; however, their roles in immune cells remain unclear.. In this study, using alanyl-tRNA synthetase (AARS) mutant (sti) mice with neurodegenerative disorder, we investigated the effect of translational fidelity in immune cells.. Dysfunctional AARS caused disorders in immune cell responses and cellularity.

    Injection:

    Article Title: Defects in aminoacyl-tRNA synthetase cause partial B and T cell immunodeficiency
    Article Snippet: In vivo homeostatic proliferation assay Naïve CD44 lo LN T cells were harvested from congenic CD45.1 + B6 mice and CD45.2 + sti mice by cell sorting using BioMag goat anti-mouse IgG beads (Qiagen). .. CD45.1 + WT and CD45.2 + sti T cells labeled with CFSE fluorescent dye (Invitrogen) were mixed at a 1:1 ratio, and a total of 1 × 10 6 naïve T cells were adoptively transferred into lymphopenic RAG1 –/– host mice via intravenous injection. ..

    Article Title: Defects in aminoacyl-tRNA synthetase cause partial B and T cell immunodeficiency.
    Article Snippet: Aminoacyl-tRNA synthetases (ARSs) are emerging as important regulators in various immune diseases; however, their roles in immune cells remain unclear.. In this study, using alanyl-tRNA synthetase (AARS) mutant (sti) mice with neurodegenerative disorder, we investigated the effect of translational fidelity in immune cells.. Dysfunctional AARS caused disorders in immune cell responses and cellularity.

    Staining:

    Article Title: In vitro study of interactions between silicon-containing nanoparticles and human peripheral blood leukocytes.
    Article Snippet: The effects of silicon dioxide-based nanoparticles on the viability and proliferative activity of human peripheral blood cultured lymphocytes were studied.. All nanoparticles in a concentration of 100 μg/ml produced a signifi cant cytotoxic effect, its intensity depending on particles’ structure: SiO2 nanoparticles were least toxic, while Ce3 +-intercaled montmorillonite nanoparticles were most toxic.. The cells died mainly by apoptosis and postapoptotic necrosis.

    CCK-8 Assay:

    Article Title: Mechanism of monoclonal antibody-coupled Staphylococcus superantigen-A induced apoptosis in human bladder cancer cells.
    Article Snippet: To investigate the mechanism of apoptosis induced by BDI-1 monoclonal antibody (MAb) coupled Staphylococcal superantigen-A (SEA) in human bladder cancer cell line BIU-87.. Human PBMC (effector cells) mediated cytotoxic killing of BIU-87 cells (target cells) was studied by culturing the BIU-87 cells in the presence of effector cells plus medium after their activation by treatment with SEA-targeted by MAb, SEA alone or vehicle (control).. Proliferation and apoptosis of BIU-87 cells was measured after the treatments.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mechanism of monoclonal antibody-coupled Staphylococcus superantigen-A induced apoptosis in human bladder cancer cells.
    Article Snippet: To investigate the mechanism of apoptosis induced by BDI-1 monoclonal antibody (MAb) coupled Staphylococcal superantigen-A (SEA) in human bladder cancer cell line BIU-87.. Human PBMC (effector cells) mediated cytotoxic killing of BIU-87 cells (target cells) was studied by culturing the BIU-87 cells in the presence of effector cells plus medium after their activation by treatment with SEA-targeted by MAb, SEA alone or vehicle (control).. Proliferation and apoptosis of BIU-87 cells was measured after the treatments.

    Suspension:

    Article Title: Integrating gene demethylation and immune modulation: PD-1 nanovesicles as a dual-action therapy for NSCLC
    Article Snippet: .. The ThermoFisher CFSE fluorescent dye was diluted 1:1000, added to the PBMC suspension, and gently mixed. ..



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